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Updated: Apr 26, 2026
Bilateral Renal Ischemia-Reperfusion Model for Acute Kidney Injury in Mice
Published on: February 2, 2024
Mouse models and methods for studying human disease, acute kidney injury (AKI)
Ganesan Ramesh1, Punithavathi Ranganathan
1Department of Medicine and Vascular Biology Center, CB-3330, Georgia Health Sciences University, 1459 Laney-Walker Blvd, Augusta, GA, 30912, USA, gramesh@gru.edu.
Abstract:
Acute kidney injury (AKI) is serious complication in hospitalized patients with high level of mortality. There is not much progress made for the past 50 years in reducing the mortality rate despite advances in understanding disease pathology. Using variety of animal models of acute kidney injury, scientist studies the pathogenic mechanism of AKI and to test therapeutic drugs, which may reduce renal injury. Among them, renal pedicle clamping and cisplatin induced nephrotoxicity in mice are most prominently used, mainly due to the availability of gene knockouts to study specific gene functions, inexpensive and availability of the inbred strain with less genetic variability. However, ischemic mouse model is highly variable and require excellent surgical skills to reduce variation in the observation. In this chapter, we describe a detailed protocol of the mouse model of bilateral renal ischemia-reperfusion and cisplatin induced nephrotoxicity. We also discuss the protocol for the isolation and analysis of infiltrated inflammatory cell into the kidney by flow cytometry. Information provided in this chapter will help scientist who wants to start research on AKI and want to establish the mouse model for ischemic and toxic kidney injury.
Insights
This study details protocols for establishing mouse models of acute kidney injury (AKI), including ischemia-reperfusion and cisplatin-induced nephrotoxicity. These models aid in understanding AKI mechanisms and testing potential therapies.
Area of Science:
- Nephrology
- Translational Medicine
- Animal Models
Background:
- Acute kidney injury (AKI) is a critical condition in hospitalized patients with high mortality rates.
- Despite advances in pathology, AKI mortality has seen limited reduction over 50 years.
- Animal models are crucial for studying AKI pathogenesis and evaluating therapeutic interventions.
Purpose of the Study:
- To provide detailed protocols for establishing mouse models of AKI.
- To enable research into the pathogenic mechanisms of ischemic and toxic kidney injury.
- To facilitate the development of novel therapeutic strategies for AKI.
Main Methods:
- Detailed protocol for bilateral renal ischemia-reperfusion injury in mice.
- Detailed protocol for cisplatin-induced nephrotoxicity in mice.
- Method for isolation and flow cytometric analysis of kidney-infiltrated inflammatory cells.
Main Results:
- Established and validated protocols for two prominent AKI mouse models.
- Demonstrated methods for analyzing inflammatory cell infiltration in kidney injury.
- Provided a comprehensive guide for researchers new to AKI modeling.
Conclusions:
- The described protocols offer a reliable foundation for AKI research using mouse models.
- Accurate modeling is essential for advancing the understanding and treatment of AKI.
- These methods support the investigation of inflammatory processes in kidney injury.
Related Concept Videos
01:30Acute Kidney Injury IV: Diagnostic Studies and Prevention
01:22Acute Kidney Injury I: Introduction
01:29Acute Kidney Injury II: Pathophysiology