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Calcium phosphate-mediated transfection alters metallothionein gene expression in response to Cd2+ and Zn2+
R Foster1, P E Olsson, L Gedamu
1Department of Biological Sciences, University of Calgary, Alberta, Canada.
Molecular and Cellular Biology
|September 1, 1989
Abstract:
The level of expression of a transfected metallothionein (MT)-IGcat fusion gene in response to cadmium differed from that of the endogenous MT-IG gene. Atomic absorption analysis indicated that the total cellular content of cadmium and zinc increased upon calcium phosphate-mediated transfection. Thus, changes in the influx/efflux of metals may regulate the level of MT gene expression.