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Immunological and chemical characterization of hamster brain polyribosomes-cytomatrix complexes
I Bouhtiauy1, Y Choukri, C Turpin
1Départment de chimie et biochimie, Université de Moncton, Canada.
Neurochemical Research
|July 1, 1989
Abstract:
We have studied the cytoskeletal nature of a brain subcellular fraction previously shown to contain polyribosomes. We have identified the major proteins of this fraction by electrophoretic comparison to a standard cytoskeletal fraction and by immunodetection. These methods have shown the presence of actin, glial fibrillary acidic protein, and neurofilament triplet proteins. We have also studied the effect of various ions and nonionic detergents on the stability of this structure. It was stable in presence of Triton X-100 up to 2% but disrupted by 200 mM K+ acetate.