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Examining thiosulfate-driven autotrophic denitrification through respirometry.

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|July 29, 2014
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Summary

This study characterizes sulfide-oxidizing nitrate-reducing (SO-NR) cultures using anoxic respirometry. The research determined the stoichiometry of thiosulfate oxidation coupled with denitrification and biomass growth.

Keywords:
Biotrickling filtersDesulfurizationRespirometryStoichiometrySulfur-oxidizing nitrogen-reducing cultures

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Area of Science:

  • Environmental microbiology
  • Biogeochemical processes

Background:

  • Biogas desulfurization utilizes sulfide-oxidizing nitrate-reducing (SO-NR) cultures.
  • Understanding SO-NR microbial physiology is crucial for optimizing biogas treatment.

Purpose of the Study:

  • To characterize SO-NR mixed cultures from a biogas desulfurization biotrickling filter.
  • To determine the stoichiometry of thiosulfate oxidation coupled with denitrification and biomass growth.
  • To validate respirometric methods using a pure culture of Thiobacillus denitrificans.

Main Methods:

  • Anoxic respirometry was employed to study microbial activity.
  • Biomass growth yield and electron acceptor consumption ratios were measured.
  • Thiosulfate oxidation and denitrification rates were assessed under varying conditions.

Main Results:

  • Nitrite was identified as an intermediate in nitrate reduction.
  • No competitive inhibition was observed between electron acceptors.
  • Specific nitrite uptake rates were higher in the SO-NR mixed culture compared to T. denitrificans pure culture.
  • The stoichiometry of the two-step denitrification mechanism was solved.

Conclusions:

  • Anoxic respirometry is effective for characterizing SO-NR cultures.
  • The SO-NR mixed culture exhibits efficient nitrite uptake.
  • This study provides novel stoichiometric insights into thiosulfate-driven denitrification with biomass growth.