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Updated: Apr 26, 2026

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
Published on: April 21, 2022
Functional sperm testing and the role of proteomics in the evaluation of male infertility
Yagil Barazani1, Ashok Agarwal1, Edmund S Sabanegh1
1Department of Urology and the Andrology Center, Glickman Urological and Kidney Institute, Cleveland Clinic, Cleveland, OH.
Abstract:
The limitations of conventional semen analysis testing in the diagnosis and management of male factor infertility have been well documented. A number of more sophisticated assays including measurements of sperm deoxyribonucleic acid (DNA) fragmentation rates, seminal oxidative stress, and antioxidant capacity have been increasingly used in the evaluation of male infertility. Moreover, in the past few years, tremendous advancements in the burgeoning field of sperm proteomics promise to revolutionize the andrologist's diagnostic armamentarium, as will be discussed in this review.
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