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miR-342-3p suppresses proliferation, migration and invasion by targeting FOXM1 in human cervical cancer

Xu-Ri Li1, Hui-Jun Chu2, Teng Lv2

  • 1Department of Obstetrics and Gynecology, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, China; Department of Gynecology and Obstetrics, The Affiliated Hiser Medical Group of Qingdao University Medical College, Qingdao, China.

FEBS Letters
|July 29, 2014
PubMed

Insights

MicroRNA-342-3p (miR-342-3p) targets the oncogenic factor Forkhead box M1 (FOXM1) in cervical cancer. Restoring miR-342-3p inhibits cancer cell growth, while FOXM1 promotes it.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Forkhead box M1 (FOXM1) is a known oncogenic transcription factor implicated in cell proliferation, angiogenesis, migration, and invasion.
  • MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and can act as tumor suppressors or oncogenes.
  • Dysregulation of FOXM1 and miRNAs is frequently observed in various cancers, including cervical cancer.

Purpose of the Study:

  • To investigate the regulatory relationship between miR-342-3p and FOXM1 in human cervical cancer.
  • To elucidate the functional role of miR-342-3p in cervical cancer progression.
  • To determine the impact of FOXM1 on the biological behavior of cervical cancer cells.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to assess miR-342-3p and FOXM1 expression levels in cervical cancer tissues and cell lines.
  • Bioinformatic analysis and luciferase reporter assays to confirm direct targeting of FOXM1 by miR-342-3p.
  • In vitro functional assays (cell proliferation, migration, invasion assays) to evaluate the effects of miR-342-3p and FOXM1 overexpression.
  • Western blotting to analyze protein expression levels.

Main Results:

  • miR-342-3p and its host gene EVL were significantly downregulated in human cervical cancer tissues compared to adjacent normal tissues.
  • FOXM1 was upregulated in cervical cancer tissues and exhibited an inverse correlation with miR-342-3p levels.
  • Overexpression of miR-342-3p suppressed cervical cancer cell proliferation, migration, and invasion.
  • Overexpression of FOXM1 reversed the inhibitory effects of miR-342-3p on cervical cancer cell phenotypes.

Conclusions:

  • miR-342-3p directly targets and represses FOXM1 expression in cervical cancer.
  • miR-342-3p functions as a tumor suppressor by inhibiting proliferation, migration, and invasion in cervical cancer.
  • The miR-342-3p/FOXM1 axis represents a potential therapeutic target for cervical cancer treatment.
Keywords:
Cervical cancerEVLForkhead Box M1miR-342-3p

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