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Updated: Apr 26, 2026

Fatty Acid 13C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers
Published on: April 17, 2018
Untargeted fatty acid profiles based on the selected ion monitoring mode
Liangxiao Zhang1, Peiwu Li2, Xiaoman Sun3
1Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China; Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China; Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan 430062, China.
Abstract:
Fatty acids are potential biomarkers of some diseases and also key markers and quality parameters of different dietary fats and related products. Thus, untargeted fatty acid profiles are important in the study of dietary fat quality and fat-related diseases, as well as in other fields such as bioenergy. In addition, accurate identification of unknown components is a technological breakthrough for the selected ion monitoring (SIM) mode for untargeted profiles. In this study, we developed untargeted fatty acid profiles based on SIM. We also investigated mass spectral characteristics and equivalent chain lengths (ECL) to eliminate the influence of non-FAMEs for identifying fatty acids in samples. As an application example, fatty acid profiles were used to classify three edible vegetable oils. The results indicated that SIM-based untargeted fatty acid profiles could yield accurate qualitative and quantitative results for more fatty acids and benefit related studies of metabolite profiles.
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