Related Experiment Video
Updated: Apr 26, 2026
Intravital Two-Photon Imaging of Touch Sensory Axon Morphology in Mouse Skin
Published on: December 30, 2025
Acute UVB-induced epidermal changes assessed by multiphoton laser tomography
M J Koehler1, K Kellner, U-C Hipler
1Department of Dermatology, SRH Waldklinikum Gera, Gera, Germany; Department of Dermatology, University Hospital Jena, Jena, Germany.
Background:
In vivo multiphoton tomography (MPT) of human skin has become a valuable tool for non-invasive examination of morphological and biophysical skin properties and their alterations. So far, skin changes after UVB irradiation were mainly evaluated clinically and histologically. The present study aimed at non-invasive imaging of histological changes during acute UVB irradiation by multiphoton laser tomography.
Methods:
In 10 volunteers, five areas were irradiated once with an erythematous UVB dose. Multiphoton measurements were performed four times, i.e. before irradiation (baseline), and 24, 48 and 72 h after irradiation, respectively. The data were evaluated for changes of epidermal pleomorphy, spongiosis, pigmentation and thickness.
Results:
The four parameters were altered significantly by acute UVB irradiation, i.e. epidermal pleomorphy, spongiosis, pigmentation and thickness increased within 72 h after irradiation.
Conclusion:
Thus, the study has shown that typical epidermal changes induced by acute UVB irradiation can be evaluated by MPT.
