Quantitative comparison of tumor delivery for multiple targeted nanoparticles simultaneously by multiplex ICP-MS
Andrew Elias1, Samuel H Crayton1, Robert Warden-Rothman2
11] Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia PA, 19104 USA [2].
Scientific Reports
|July 29, 2014
Summary
Researchers developed a novel lanthanide-doped nanoparticle method for simultaneously comparing multiple targeted nanoparticles in vivo. This technique overcomes limitations of traditional animal testing for evaluating nanoparticle efficacy and biodistribution.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Materials Science
Background:
- The exponential growth of targeted nanoparticles for disease biomarkers necessitates efficient evaluation methods.
- In vivo testing of numerous nanoparticle formulations is often infeasible due to cost and variability.
- In vitro assays cannot fully predict in vivo nanoparticle performance.
Purpose of the Study:
- To develop a method for simultaneous quantitative comparison of multiple targeted nanoparticles in vivo.
- To enable efficient screening of nanoparticle formulations for therapeutic applications.
Main Methods:
- Development of lanthanide-doped superparamagnetic iron oxide (SPIO) nanoparticles, each with a unique lanthanide dopant and targeting ligand.
- Simultaneous intravenous injection of differentially doped SPIO nanoparticles into tumor-bearing mice.
- Quantification of SPIO compositions in blood and tumor samples using inductively coupled plasma mass spectroscopy (ICP-MS).
Main Results:
- Successful synthesis of SPIO nanoparticles with distinct lanthanide dopants and targeting ligands.
- Demonstration of simultaneous in vivo tracking and quantification of multiple SPIO compositions.
- Orthogonal assessment of nanoparticle biodistribution and tumor accumulation.
Conclusions:
- Lanthanide-doped nanoparticles offer a powerful platform for the simultaneous in vivo evaluation of targeted nanoparticle formulations.
- This method significantly enhances the efficiency and accuracy of nanoparticle screening for preclinical research.
- The approach facilitates the selection of optimal nanoparticle candidates for further development.
