Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

1.9K
The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
1.9K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

2.1K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.1K
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

3.0K
Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
3.0K
Electrospray Ionization (ESI) Mass Spectrometry01:12

Electrospray Ionization (ESI) Mass Spectrometry

2.6K
Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
2.6K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

5.7K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
5.7K
Mass Analyzers: Overview01:13

Mass Analyzers: Overview

2.0K
The mass analyzer is a crucial component of the mass spectrometer. In the ionization chamber, the vaporized sample is bombarded with a high-energy electron beam to generate a radical cation and further fragment into neutral molecules, radicals, and cations. A series of negatively charged accelerator plates accelerate the cations into the mass analyzer. The mass analyzer separates ions according to their mass-to-charge (m/z) ratios and then directs them to the detector. The common types of mass...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proteomic Impact of Peripheral Expression of Mutant Huntingtin in <i>C. elegans</i>.

Journal of proteome research·2026
Same author

Toward Metabolomics Analyses With Combined Capillary Vibrating Sharp-Edge Spray Ionization and Atmospheric Pressure Chemical Ionization.

Rapid communications in mass spectrometry : RCM·2026
Same author

Inhalation exposure to surrogate military burn pit emissions impairs systemic microvascular function: linking pulmonary insult and diverse peripheral responses.

Particle and fibre toxicology·2026
Same author

Structural Characterization of Calcium-Dependent Calmodulin-Calmidazolium Binding using Capillary Vibrating Sharp-Edge Spray-based Native Mass Spectrometry and In-Droplet Hydrogen Deuterium Exchange Mass Spectrometry.

bioRxiv : the preprint server for biology·2026
Same author

Integrating Simple Microfluidics Design/Fabrication with a Novel Ionization Source for Time-Resolved Chemical Reaction Studies by Mass Spectrometry.

ACS omega·2026
Same author

3D-printed serpentine columns for size-exclusion chromatography with mass spectrometric detection.

Analytica chimica acta·2025

Related Experiment Video

Updated: Apr 26, 2026

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
08:56

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Published on: November 22, 2024

1.6K

A new ion mobility-linear ion trap instrument for complex mixture analysis.

Gregory C Donohoe1, Hossein Maleki, James R Arndt

  • 1C. Eugene Bennett Department of Chemistry, West Virginia University , Morgantown, West Virginia 26506, United States.

Analytical Chemistry
|July 29, 2014
PubMed
Summary
This summary is machine-generated.

A novel instrument combines ion mobility spectrometry with mass spectrometry for sensitive analysis of complex mixtures. This technology offers high reproducibility and unique fragmentation capabilities for proteomics and metabolomics.

More Related Videos

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

3.8K
T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis
16:40

T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis

Published on: July 31, 2010

27.2K

Related Experiment Videos

Last Updated: Apr 26, 2026

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
08:56

Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry

Published on: November 22, 2024

1.6K
Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments
08:40

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Published on: January 20, 2022

3.8K
T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis
16:40

T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis

Published on: July 31, 2010

27.2K

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Physical Chemistry

Background:

  • Complex mixtures in proteomics and metabolomics require advanced analytical techniques.
  • Existing methods may lack sensitivity, reproducibility, or fragmentation capabilities.
  • Ion mobility spectrometry coupled with mass spectrometry (IMS-MS) offers enhanced separation.

Purpose of the Study:

  • To demonstrate a new instrument coupling a low-pressure drift tube with a linear ion trap mass spectrometer.
  • To showcase its utility for complex mixture analysis, particularly in proteomics and metabolomics.
  • To highlight the benefits of combined ion mobility separation and mass spectrometry.

Main Methods:

  • Utilizing a low-pressure drift tube for gas-phase ion separation.
  • Employing a linear ion trap mass spectrometer for ion detection and fragmentation.
  • Implementing multistage tandem mass spectrometry (MS(n)) with collision-induced dissociation (CID) and electron transfer dissociation (ETD).

Main Results:

  • Achieved attomole-level detection limits for mobility-selected peptide ions.
  • Demonstrated unique ion fragmentation capabilities within the drift tube and ion trap.
  • Showcased high reproducibility enabling pixel-by-pixel comparison of 2D IMS-MS data sets without alignment.

Conclusions:

  • The coupled low-pressure drift tube and linear ion trap MS system provides high sensitivity and unique fragmentation for complex mixtures.
  • The instrument's reproducibility facilitates direct comparison of analytical data.
  • This technology shows significant promise for applications in proteomics and metabolomics.