Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Stem Cell Culture01:17

Stem Cell Culture

4.5K
Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
4.5K
Adult Stem Cells01:33

Adult Stem Cells

27.7K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
27.7K
Embryonic Stem Cells00:58

Embryonic Stem Cells

25.7K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
25.7K
Embryonic Stem Cells00:57

Embryonic Stem Cells

4.5K
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
4.5K
Source And Potency Of Stem Cells01:27

Source And Potency Of Stem Cells

5.0K
Stem cells are undifferentiated cells with extensive self-renewal properties that help them maintain their population during the fetal and adult stages of life. They can specialize in all cell types of the human body. However, their differential potential may vary and can be classified into five types. Stem cells can be (1) Totipotent, (2) Pluripotent, (3) Multipotent, (4) Oligopotent, and (5) Unipotent. Each stem cell has a specific origin; the fertilized egg or zygote is a totipotent cell and...
5.0K
Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

4.5K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
4.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

IL-21 isoform transgenic mice spontaneously develop mammary tumors: possible involvement of IL-21-induced osteopontin expression in tumorigenesis.

Frontiers in immunology·2026
Same author

Correction: IL-21 isoform transgenic mice spontaneously develop mammary tumors: possible involvement of IL-21-induced osteopontin expression in tumorigenesis.

Frontiers in immunology·2026
Same author

Relative contributions of vegetation and soil properties to microbial community structure and function in alpine and subalpine meadows of the southeastern Tibetan Plateau.

Frontiers in microbiology·2026
Same author

Attentional resource allocation in the early stages of motor skill learning.

International journal of psychophysiology : official journal of the International Organization of Psychophysiology·2026
Same author

Effect of route familiarity on attentional resource allocation during real-world road driving.

Scientific reports·2026
Same author

Lifitegrast-eluting contact lens for dry eye disease: sustained release, enhanced ocular bioavailability, and robust in vitro-in vivo correlation.

Drug delivery and translational research·2026
Same journal

Hematopoietic Cell Fate Reprogramming in the Context of Pathological Processes and Translational Opportunities.

Cellular reprogramming·2026
Same journal

Liquid-Cell Interactions in the Tumor Microenvironment: Mechanisms, Biomarkers, and Therapeutic Opportunities.

Cellular reprogramming·2026
Same journal

Reprogramming Stars #28: Stem Cell-Based Approaches to Build Blood Vessels and Liver Tissue-An Interview with Dr. Lay Teng Ang.

Cellular reprogramming·2026
Same journal

Isolation of Buffalo (<i>Bubalus bubalis</i>) Theca Cells: A Comparison Among Three Protocols.

Cellular reprogramming·2026
Same journal

The Involvement of Gut Microbiota and Their Key Metabolites in Regulating Fetal Development via the Gut-Placental Axis.

Cellular reprogramming·2026
Same journal

Reprogramming Stars #27: Drawing Inspiration from Reprogramming to Guide Stem Cell Differentiation: An Interview with Dr. Kyle Loh.

Cellular reprogramming·2026
See all related articles

Related Experiment Video

Updated: Apr 26, 2026

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
08:24

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Published on: February 28, 2017

6.5K

Characterization of amniotic stem cells.

Chika Koike1, Kaixuan Zhou, Yuji Takeda

  • 11 Department of Regenerative Medicine, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama , Toyama, 930-0194, Japan .

Cellular Reprogramming
|July 29, 2014
PubMed
Summary
This summary is machine-generated.

Amniotic stem cells show potential for regenerative medicine. Researchers identified TRA-1-60 as a key marker for isolating these valuable epithelial stem cells.

More Related Videos

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

8.6K
In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
10:00

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

Published on: November 28, 2019

7.5K

Related Experiment Videos

Last Updated: Apr 26, 2026

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System
08:24

The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Published on: February 28, 2017

6.5K
Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

8.6K
In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
10:00

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

Published on: November 28, 2019

7.5K

Area of Science:

  • Stem cell biology
  • Developmental biology
  • Regenerative medicine

Background:

  • Amniotic membrane cells are embryo-derived and possess multidifferentiation potential, making them a promising stem cell source.
  • Limited molecular characterization and unclear isolation markers hinder the use of amnion-derived stem cells.

Purpose of the Study:

  • To characterize amnion-derived epithelial and mesenchymal cells as stem cells.
  • To identify efficient markers for isolating amnion-derived stem cells.

Main Methods:

  • Flow cytometry, immunocytochemistry, and quantitative PCR were used to analyze stemness marker expression.
  • Comparison of stemness gene expression in amnion cells versus fibroblasts, bone marrow-derived mesenchymal stem cells (MSCs), and induced pluripotent stem cells (iPSCs).

Main Results:

  • Amnion epithelial cells expressed CD133, CD271, and TRA-1-60; mesenchymal cells expressed CD44, CD73, CD90, and CD105.
  • Both cell types expressed stemness markers Oct3/4, Sox2, Klf4, and SSEA4.
  • Amnion-derived cells showed higher expression of Oct3/4, Nanog, and Klf4 compared to bone marrow-derived MSCs. TRA-1-60 positive cells exhibited higher expression of these genes.

Conclusions:

  • TRA-1-60 is identified as a potential marker for isolating amnion epithelial stem cells.
  • Amnion-derived cells possess significant stemness properties, comparable or superior to MSCs and iPSCs.