Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Neoadjuvant immunotherapy for resectable cutaneous melanoma: a nationwide population-based cohort.

Journal of the National Cancer Institute·2026

Same author

Impact of Boric Acid on the Stability of Hydrogen Peroxide in Aqueous Solution.

ACS omega·2026

Same author

Time of day of neoadjuvant immune checkpoint inhibitor administration is associated with survival but not pathological response in resectable stage III-IV melanoma: A retrospective cohort study.

European journal of cancer (Oxford, England : 1990)·2026

Same author

Interpreting the effects of DNA polymerase variants at the structural level.

Molecular oncology·2026

Same author

NeoCircle: pre- and post-operative circulating tumor DNA dynamics predicts survival in neoadjuvant-treated early breast cancer.

EMBO molecular medicine·2026

Same author

The Effects of Sun Exposure and Pigmentation Phenotype on Prognosis in Metastatic Melanoma.

Acta dermato-venereologica·2026

Search research articles

Related Experiment Video

Updated: Apr 26, 2026

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Published on: February 27, 2015

Discovery-based protein expression profiling identifies distinct subgroups and pathways in leiomyosarcomas.

Ufuk Kirik¹, Karin Hansson¹, Morten Krogh²

¹Department of Immunotechnology, Lund University, Lund, Sweden.

Molecular Cancer Research : MCR

|July 30, 2014

Summary

Undifferentiated pleomorphic sarcomas (UPS) and leiomyosarcomas share similar protein expression, suggesting a common mesenchymal stem cell origin. This finding aids in classifying these challenging soft tissue sarcomas.

More Related Videos

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Published on: July 22, 2020

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

Published on: October 19, 2014

Related Experiment Videos

Last Updated: Apr 26, 2026

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Published on: February 27, 2015

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Published on: July 22, 2020

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

From a 2DE-Gel Spot to Protein Function: Lesson Learned From HS1 in Chronic Lymphocytic Leukemia

Published on: October 19, 2014

Area of Science:

Oncology
Proteomics
Molecular Biology

Background:

Soft tissue sarcomas (STS) present diagnostic challenges due to complex karyotypes and lack of clear chromosomal aberrations.
Accurate histopathologic and molecular classification of STS remains difficult.

Purpose of the Study:

To investigate protein expression patterns in human STS subtypes using proteomics.
To identify molecular subgroups within pleomorphic STS and leiomyosarcomas.
To compare protein expression between leiomyosarcomas and undifferentiated pleomorphic sarcomas (UPS).

Main Methods:

Two-dimensional gel proteomics was employed to analyze protein expression across various human STS subtypes.
In-depth mass spectrometry characterized proteome profiles of pleomorphic STS specimens.
Bioinformatic pathway analysis examined differentially regulated biologic nodes.

Main Results:

Distinct protein expression patterns identified subgroups within leiomyosarcomas.
Pathway analysis revealed differential regulation of apoptosis, cytoskeleton remodeling, and telomere regulation.
Leiomyosarcomas and UPS exhibited similar protein expression profiles compared to pleomorphic leiomyosarcomas.

Conclusions:

UPS tumors likely originate from a similar lineage as leiomyosarcomas.
These sarcomas may arise from varying differentiation stages of mesenchymal stem cells towards smooth muscle cells.