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Updated: Apr 26, 2026

Resonance Raman Spectroscopy of Extreme Nanowires and Other 1D Systems
Published on: April 28, 2016
Temperature-sensitive gating of hCx26: high-resolution Raman spectroscopy sheds light on conformational changes
Ann-Kathrin Kniggendorf1, Merve Meinhardt-Wollweber2, Xiaogang Yuan1
1Hanover Centre for Optical Technologies, Gottfried-Wilhelm-Leibniz Universität Hannover, Nienburger Str. 17, 30167 Hanover, Germany.
Abstract:
The temperature-sensitive gating of human Connexin 26 (hCx26) was analyzed with confocal Raman microscopy. High-resolution Raman spectra covering the spectral range between 400 and 1500 rel. cm(-1) with a spectral resolution of 1 cm(-1) were fully annotated, revealing notable differences between the spectrum recorded from solubilized hCx26 in Ca(2+)-buffered POPC at 10°C and any other set of protein conditions (temperature, Ca(2+) presence, POPC presence). Spectral components originating from specific amino acids show that the TM1/EL1 parahelix and probably the TM4 trans-membrane helix and the plug domain are involved in the gating process responsible for fully closing the hemichannel.
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