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Updated: Apr 26, 2026

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
Published on: April 14, 2015
Biomolecule-to-fluorescent-color encoder: modulation of fluorescence emission via DNA structural changes
Takahiro Nishimura1, Yusuke Ogura2, Kenji Yamada1
1Graduate School of Medicine, Osaka University, Suita, Osaka, 5650871, Japan.
Abstract:
A biomolecule-to-fluorescent-color (B/F) encoder for optical readout of biomolecular information is proposed. In the B/F encoder, a set of fluorescence wavelengths and their intensity levels are used for coding of a biomolecular signal. A hybridization chain reaction of hairpin DNAs labeled with fluorescent reporters was performed to generate the fluorescence color codes. The fluorescence is modulated via fluorescence resonance energy transfer, which is controlled by DNA structural changes. The results demonstrate that fluorescent color codes can be configured based on two wavelengths and five intensities using the B/F encoder, and the assigned codes can be retrieved via fluorescence measurements.
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