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Updated: Apr 26, 2026

Simultaneous Label-Free Autofluorescence Multi-Harmonic Microscopy
Published on: August 29, 2025
Multi-modal label-free imaging based on a femtosecond fiber laser
Ruxin Xie1, Jue Su1, Eric C Rentchler2
1Department Electrical Engineering & Computer Science, University of Kansas, Lawrence, KS, 66045, USA.
Abstract:
We demonstrate multi-mode microscopy based on a single femtosecond fiber laser. Coherent anti-Stokes Raman scattering (CARS), stimulated Raman scattering (SRS) and photothermal images can be obtained simultaneously with this simplified setup. Distributions of lipid and hemoglobin in sliced mouse brain samples and blood cells are imaged. The dependency of signal amplitude on the pump power and pump modulation frequency is characterized, which allows to isolate the impact from different contributions.
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