Related Experiment Video
Updated: Apr 26, 2026

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System
Published on: January 5, 2024
Prevalence and mapping of a plasmid encoding a type IV secretion system in Acinetobacter baumannii
Chih-Chin Liu1, Han-Yueh Kuo2, Chuan Yi Tang3
1Department of Bioinformatics, Chung Hua University, Hsin-Chu City, Taiwan; Department of Computer Science and Information Engineering, Providence University, Taichung County, Taiwan.
Abstract:
We investigated the prevalence of a type IV secretion system (T4SS)-bearing plasmid among clinical isolates of carbapenem-resistant Acinetobacter baumannii (CRAB) using plasmid replicon typing. The complete sequence of a T4SS-bearing plasmid, pAB_CC, isolated from A. baumannii TYTH-1 was determined, and a comparative analysis of the T4SS gene modules was performed. Of the 129 isolates studied, GR6 (repAci6) was the most common (45 of 96 isolates) and was strongly linked with the T4SS. A comparative analysis of the T4SS locus in seven plasmid genomes, including pAB_CC, pACICU2, pABKp1, pABTJ1, p1BJAB0714, p2BJAB0868, and p2ABTCDC0715, indicated that fourteen genes on these plasmids were highly conserved compared to those of the F plasmid. Additionally, the chromosomes in the seven representative isolates may be evolutionarily distinct from their intrinsic T4SS-bearing plasmids, suggesting that the two T4SS lineages emerged long before the appearance of EC II. These two lineages are now widespread in A. baumannii strains.
More Related Videos
08:37The Application of Open Searching-based Approaches for the Identification of Acinetobacter baumannii O-linked Glycopeptides
Published on: November 2, 2021
07:41Author Spotlight: A Comprehensive Protocol for Acinetobacter Biofilm Quantification, Assessment, and Visualization
Published on: August 4, 2023
Related Concept Videos
Gram-negative Bacterial Protein Secretion Systems
Bacterial Translocation and Protein Secretion
Plasmids
Regulation of Bacterial Virulence
Mechanism of Conjugation
Gene Regulation in Microbial Communities: Quorum Sensing