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Effect of handling of plasma samples on histamine radio-enzyme assay
R J Harvima1, I T Harvima, E O Kajander
1Department of Dermatology, University of Kuopio, Finland.
The International Journal of Biochemistry
|January 1, 1989
Abstract:
1. Freeze-thawing of plasma samples increased the histamine level at physiological histamine concentrations as analyzed by radio-enzyme assay, but not by high performance liquid chromatography. 2. Heating of plasma samples decreased the histamine levels. 3. Enzymatic or non-enzymatic formation of histamine during handling of plasma samples was not detected. 4. Some albumin preparations contain histamine.