Related Experiment Video
Updated: Jan 24, 2026

Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor Cells
Published on: August 25, 2021
Low-heat treated autograft versus allograft for intercalary reconstruction of malignant bone tumors
Ilkyu Han1, June Hyuk Kim, Hwan-Seong Cho
1Department of Orthopaedic Surgery, Seoul National University Hospital, Seoul, Korea.
Introduction:
Low-heat treated autograft may have an advantage over allograft in that they provide a size-matched and biologically compatible graft.
Materials And Methods:
We compared the graft survival and complications between the low-heat treated autografts (n = 13) and allografts (17) for intercalary reconstruction of malignant bone tumors. Average age was 25 years with follow-up of 6.6 years (1.8-12.5). For low-heat treatment, the resected bone was treated in saline at 65°C for 30 min. Nineteen grafts were fixed with plates and 13 with intramedullary nails.
Results:
Until last follow-up, five low-heat treated autografts (38%) and one allograft (6%) had been removed, with allografts showing a trend toward better survival on Kaplan-Meier analysis (P = 0.293). Complications were more common in low-heat treated autograft group [10/13 (77%) vs. 8/17 (47%), P = 0.098)]. Low-heat-treated autograft resulted in significantly higher rate of nonunion [11/26 (42%) vs. 5/34 (15%), P = 0.017] and required higher number of operations to treat nonunion (P = 0.004). Rates of graft fracture [3/13 (23%) vs. 2/17 (12%), P = 0.713)] or infection [2/13 (15%) vs. 1/17 (6%), P = 0.565)] were similar.
Conclusion:
Our data suggest that intercalary allograft has better survival and fewer complications than the low-heat treated autograft.
More Related Videos
Related Concept Videos
Quantifying Heat
Specific Heat
For example, increasing the temperature of one gram of water by 1°C requires one calorie of heat energy and can be written as 1 cal/g-°C, or...
Heat Flow and Specific Heat
Heating and Cooling Curves
For instance, the addition of heat raises the temperature of a solid; the amount of heat absorbed depends on the heat capacity of the solid (q = mcsolidΔT). According to thermochemistry, the relation between the amount of heat absorbed or released by a substance, q, and its...
Heat and Free Expansion
Heat Engines
Whenever we consider heat engines (and associated devices such as refrigerators and heat pumps), we do not use the standard sign convention for heat and work. For convenience, we assume that the symbols Qh, Qc, and W represent only the amounts of heat transferred...

