Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

Overview of Microscopy Techniques

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Progress of artificial intelligence technology in pneumoconiosis imaging diagnosis and disease burden assessment].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases·2026

Same author

Final overall survival analysis and exploratory biomarker study from JUPITER-06: a randomized phase III trial of toripalimab plus chemotherapy in advanced esophageal squamous-cell carcinoma.

Annals of oncology : official journal of the European Society for Medical Oncology·2026

Same author

Impact of systemic therapy on clinical T1 small-cell neuroendocrine carcinoma of the bladder.

ESMO open·2024

Same author

Assessing morinidazole for surgical site infection in class III wounds prevention: a multi-centre, randomized, single-blind, parallel-controlled study.

The Journal of hospital infection·2024

Same author

[Investigation and analysis on the detection of IgG antibodies against the rubella virus among rural childbearing-age women in preconception period in Yunnan Province from 2013 to 2019].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2024

Same author

[Kounis syndrome complicated with Takotsubo cardiomyopathy: a case report].

Zhonghua nei ke za zhi·2023

Search research articles

Related Experiment Video

Updated: Apr 26, 2026

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy

Published on: January 7, 2019

Multimodal microscopy using 'half and half' contact mode and ultrasonic force microscopy.

M S Skilbeck¹, A J Marsden, G Cao

¹Department of Physics, University of Warwick, Coventry CV4 7AL, UK.

|July 31, 2014

Summary

This study introduces a novel multimodal atomic force microscopy (AFM) technique combining ultrasonic force microscopy (UFM) and contact mode scanning. This method enables simultaneous nanoscale mapping of nanomechanical, friction, and conductivity properties for advanced material characterization.

More Related Videos

Contact Mode Atomic Force Microscopy as a Rapid Technique for Morphological Observation and Bacterial Cell Damage Analysis

Contact Mode Atomic Force Microscopy as a Rapid Technique for Morphological Observation and Bacterial Cell Damage Analysis

Published on: June 30, 2023

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Published on: December 1, 2023

Related Experiment Videos

Last Updated: Apr 26, 2026

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy

Published on: January 7, 2019

Contact Mode Atomic Force Microscopy as a Rapid Technique for Morphological Observation and Bacterial Cell Damage Analysis

Contact Mode Atomic Force Microscopy as a Rapid Technique for Morphological Observation and Bacterial Cell Damage Analysis

Published on: June 30, 2023

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Published on: December 1, 2023

Area of Science:

Materials Science
Nanotechnology
Surface Science

Background:

Characterizing multifunctional nanostructured materials demands techniques for simultaneous multi-property mapping at the nanoscale.
Existing methods often lack the resolution or multi-modal capability required for complex nanomaterials.

Purpose of the Study:

To develop and demonstrate a multimodal atomic force microscopy (AFM) approach for simultaneous nanoscale property mapping.
To combine nanomechanical, friction, and conductivity measurements for comprehensive material analysis.

Main Methods:

Utilized a 'half and half' approach, rapidly alternating between ultrasonic force microscopy (UFM) and contact mode scanning within AFM.
Acquired simultaneous contact mode (friction, conductivity) and UFM (nanomechanical) data at each pixel for high-resolution imaging.
Applied the technique to graphene on copper, single-walled carbon nanotubes (SWNTs) on silicon oxide, and graphene epoxy composites.

Main Results:

Successfully demonstrated simultaneous mapping of nanomechanical, friction, and conductivity properties at the nanoscale.
The 'half and half' method allowed friction force measurement without topographical cross-talk.
Ultrasonic force microscopy's superlubricity enabled standard contact mode imaging of delicate samples like SWNTs.

Conclusions:

The developed multimodal AFM technique is effective for characterizing complex nanostructured materials.
This approach offers significant advantages for analyzing delicate nanomaterials and composite systems.
It provides a powerful tool for advancing the design and fabrication of multifunctional nanostructured materials.