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Updated: Apr 26, 2026

One-step Protocol for Evaluation of the Mode of Radiation-induced Clonogenic Cell Death by Fluorescence Microscopy
Published on: October 23, 2017
Alterations in gene expression during radiation-induced mitotic catastrophe in HeLa Hep2 cells
Theres Lindgren1, Torgny Stigbrand1, Lennart Johansson2
1Department of Clinical Microbiology, Immunology, Umeå University, Umeå, Sweden.
Aim:
To explore kinetic changes in the gene expression profile during radiation-induced mitotic catastrophes.
Materials And Methods:
Gene expression changes were measured in HPV-infected HeLa Hep2 tumor cells following exposure to 5 Gy of ionizing radiation ((60)Co). Signaling pathways were explored and correlated to the biological responses linked to mitotic catastrophe.
Results:
Following irradiation a transient G2-arrest was induced. Anaphase bridge formation and centrosome hyperamplification was observed. These phenotypical changes correlated well with the observed gene expression changes. Genes with altered expression were found to be involved in mitotic processes as well as G2- and spindle assembly checkpoints. Also centrosome-associated genes displayed an increased expression.
Conclusion:
This study elucidates specific characteristics in the altered gene expression pattern induced by irradiation, which can be correlated to the events of mitotic catastrophe in HeLa Hep2 cells. Therapeutic strategies modulating these alterations might potentiate future therapy and enhance tumor cell killing.
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