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Retinal Pathophysiological Evaluation in a Rat Model

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Paraffin treasures: do they last forever?

Vera Ademà¹, Erica Torres, Francesc Solé

¹1 Laboratoris de Citogenètica i Biologia Molecular. Servei de Patologia. Hospital del Mar. Barcelona , Spain .

Biopreservation and Biobanking

|July 31, 2014

Summary

DNA quality from formalin-fixed paraffin-embedded (FFPE) tissues degrades over time. Nucleic acid extraction from older FFPE blocks yields lower quality DNA, impacting molecular analysis.

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Area of Science:

Pathology
Molecular Biology
Biorepository Science

Background:

Formalin-fixed paraffin-embedded (FFPE) tissues are crucial for diagnostics and research.
Nucleic acid extraction from aged FFPE samples presents significant challenges.
Long-term storage can compromise DNA integrity.

Purpose of the Study:

To evaluate DNA degradation in FFPE tissues over extended storage periods.
To compare DNA quality from FFPE blocks stored for different durations.
To assess the impact of storage time on DNA amplifiability for molecular analysis.

Main Methods:

Comparison of DNA quality extracted in 2002 versus 2010 from identical FFPE blocks.
Polymerase Chain Reaction (PCR) to assess the amplifiable length of nuclear DNA.
Evaluation of DNA integrity and amplifiability after years of FFPE storage.

Main Results:

DNA quality from FFPE tissues generally decreases with prolonged storage.
A significant reduction in amplifiable nuclear DNA length was observed in older samples.
Only a small proportion of FFPE samples retained comparable DNA amplifiability over time.

Conclusions:

Extended storage of FFPE tissues leads to DNA degradation, affecting molecular analysis.
For future molecular studies, nucleic acid extraction should be performed soon after the diagnostic process.
Preserving frozen samples in institutional biorepositories is recommended when possible for better nucleic acid quality.