Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

2.6K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
2.6K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

868
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
868
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

1.8K
A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
1.8K
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

1.8K
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
1.8K
Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview

1.5K
Attenuated total reflectance (ATR) infrared spectroscopy is a powerful analytical technique used to study the composition of materials. It is widely employed in chemistry, materials science, forensic science, and other fields where sample characterization is required. ATR has several advantages over traditional transmission IR spectroscopy, including the requirement of little to no sample preparation and the ability to analyze a wide range of samples.
The ATR process begins by directing a beam...
1.5K
IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations01:08

IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations

2.0K
Identical bonds within a polyatomic group can stretch symmetrically (in-phase) or asymmetrically (out-of-phase). Similar to hydrogen bonding, these vibrations also influence the shape of the IR peak. Generally, asymmetric stretching frequencies are higher than symmetric stretching frequencies. For example, primary amines exhibit two distinct IR peaks between 3300–3500 cm−1 corresponding to the symmetric and asymmetric N-H stretching, while secondary amines exhibit a single...
2.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exception From Informed Consent Community Consultation Surveys-Do Respondent Characteristics Accurately Reflect Targeted Communities?

Academic emergency medicine : official journal of the Society for Academic Emergency Medicine·2025
Same author

Temperature Dependence of the Mechanical Dissipation of Gallium Bonds for Use in Gravitational Wave Detectors.

Physical review letters·2024
Same author

Results of a Quality Improvement Initiative to Increase the Completion Rate of Electronic Health Records for Patient Encounters at a Large Urban Fire-Based Non-Transporting EMS Agency.

Prehospital emergency care·2023
Same author

Increased weight in patients with time-sensitive diagnosis is associated with longer prehospital on-scene times.

The American journal of emergency medicine·2022
Same author

Enhanced medium-range order in vapor-deposited germania glasses at elevated temperatures.

Science advances·2021
Same author

Low Mechanical Loss TiO_{2}:GeO_{2} Coatings for Reduced Thermal Noise in Gravitational Wave Interferometers.

Physical review letters·2021

Related Experiment Video

Updated: Apr 26, 2026

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems
09:57

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

6.0K

Two-tone frequency-modulation stimulated Rayleigh spectroscopy.

Gregory W Faris, Ashot Markosyan, Christina L Porter

    Optics Letters
    |August 1, 2014
    PubMed
    Summary

    We developed two-tone frequency-modulation (FM) stimulated Rayleigh spectroscopy for high-resolution measurements. This technique retains phase information, offering improved accuracy and signal quality using a single laser.

    More Related Videos

    Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis
    10:57

    Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis

    Published on: February 1, 2022

    2.9K
    Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
    09:13

    Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

    Published on: July 6, 2019

    7.2K

    Related Experiment Videos

    Last Updated: Apr 26, 2026

    Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems
    09:57

    Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

    Published on: February 10, 2020

    6.0K
    Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis
    10:57

    Real-Time, Two-Color Stimulated Raman Scattering Imaging of Mouse Brain for Tissue Diagnosis

    Published on: February 1, 2022

    2.9K
    Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering
    09:13

    Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

    Published on: July 6, 2019

    7.2K

    Area of Science:

    • Spectroscopy
    • Laser Physics
    • Physical Chemistry

    Background:

    • High spectral resolution is crucial for detailed molecular analysis.
    • Existing spectroscopic methods may lack accuracy or signal quality.
    • Frequency-modulation techniques offer enhanced sensitivity.

    Purpose of the Study:

    • To demonstrate a novel two-tone frequency-modulation (FM) stimulated Rayleigh spectroscopy method.
    • To achieve high spectral resolution and accurate pump/probe detuning.
    • To retain the phase signal, unlike in FM absorption spectroscopy.

    Main Methods:

    • Utilizing a single narrowband laser as the master oscillator.
    • Employing an electro-optic modulator for pump/probe detuning and FM sideband generation.
    • Using a double-pass two-rod Nd:YAG amplifier for high pump beam power (∼1 kW).

    Main Results:

    • Achieved high spectral resolution (∼1 MHz).
    • Demonstrated excellent pump/probe detuning accuracy.
    • Obtained near-shot-noise-limited signal-to-noise ratios.
    • Confirmed that the phase component of the stimulated thermal Rayleigh peak agrees with theoretical predictions.

    Conclusions:

    • Two-tone FM stimulated Rayleigh spectroscopy is a viable technique for high-fidelity spectral measurements.
    • The method offers significant advantages in resolution, accuracy, and signal quality.
    • Retaining the phase signal provides a more complete spectral characterization.