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Related Concept Videos

Atomic Absorption Spectroscopy: Instrumentation01:22

Atomic Absorption Spectroscopy: Instrumentation

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An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
The atomizer used in AAS can be either a flame atomizer or an...
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Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

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Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
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Atomic Emission Spectroscopy: Instrumentation01:22

Atomic Emission Spectroscopy: Instrumentation

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The instrumentation of atomic emission spectrometry (AES) involves various components, including atomization devices that convert samples into gas-phase atoms and ions. There are two main types of atomization devices: continuous and discrete atomizers.  Continuous atomizers, like plasmas and flames, introduce samples in a constant stream, while discrete atomizers inject individual samples using syringes or autosamplers. The most common discrete atomizer is the electrothermal atomizer.
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Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
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IR Spectrometers01:25

IR Spectrometers

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There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
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Instrumentation Amplifier01:25

Instrumentation Amplifier

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An electrocardiography (ECG) machine is an essential piece of medical equipment used to monitor the electrical activity of the heart. It operates by detecting small electrical changes on the skin that result from the depolarization of the heart muscle during each heartbeat. However, these signals are in the microvolt range and can be easily overwhelmed by noise or interference.
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Related Experiment Video

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SIVQ-LCM Protocol for the ArcturusXT Instrument
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Published on: July 23, 2014

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SIVQ-LCM protocol for the ArcturusXT instrument.

Jason D Hipp1, Jerome Cheng2, Jeffrey C Hanson1

  • 1Laboratory of Pathology, National Cancer Institute, National Institutes of Health.

Journal of Visualized Experiments : Jove
|August 1, 2014
PubMed
Summary

Spatially Invariant Vector Quantization-Laser Capture Microdissection (SIVQ-LCM) automates laser dissection for faster, more accurate tissue microdissection. This advanced platform enhances sample throughput in research and clinical settings.

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Genomics

Background:

  • Traditional laser capture microdissection (LCM) is user-dependent and time-consuming.
  • Automating LCM enhances speed, accuracy, and reproducibility.
  • Developing advanced, customizable laser dissection platforms is crucial for research.

Purpose of the Study:

  • To integrate Spatially Invariant Vector Quantization (SIVQ) image analysis software with the ArcturusXT laser dissection system.
  • To create a rapidly customizable and automated laser dissection platform.
  • To improve sample throughput for both animal and human tissue microdissection.

Main Methods:

  • Integration of SIVQ image analysis software with the ArcturusXT instrument.
  • Utilizing both infrared (IR) and ultraviolet (UV) lasers for precise cell or large area dissections.
  • Developing an automated workflow for laser capture microdissection.

Main Results:

  • The SIVQ-LCM methodology automates and streamlines the laser dissection process.
  • The integrated system allows for rapid and customizable dissections.
  • Increased speed, accuracy, and reproducibility in laser dissection were achieved.

Conclusions:

  • SIVQ-LCM offers a novel, automated approach to laser capture microdissection.
  • This technology facilitates efficient microdissection of diverse biological tissues.
  • The platform has significant potential for research and clinical applications, improving sample throughput.