The β-Lacta test for direct detection of extended-spectrum-β-lactamase-producing Enterobacteriaceae in urine
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Updated: Apr 26, 2026

Bacterial Detection & Identification Using Electrochemical Sensors
Published on: April 23, 2013
Salah Gallah1, Dominique Decré2, Nathalie Genel3
1Assistance Publique-Hôpitaux de Paris, HUEP, Hôpital Tenon, Department of Bacteriology-Hygiene, Paris, France.
Abstract:
With the β-Lacta test, production of extended-spectrum β-lactamases (ESBLs) was assayed in 200 urine samples showing Gram-negative bacilli during direct microscopic examination. While 168 samples tested negative, all samples yielding ESBL-producing Enterobacteriaceae after culture gave positive (n = 30) or uninterpretable (n = 2) results. The sensitivity and specificity of ESBL detection were 94% and 100%, respectively.
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