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Related Experiment Video

Updated: Apr 26, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

An EWOD-based microfluidic chip for single-cell isolation, mRNA purification and subsequent multiplex qPCR.

A Rival¹, D Jary, C Delattre

¹CEA, IRTSV, Laboratoire de Biologie à Grande Echelle, F-38054 Grenoble Cedex 9, France. xavier.gidrol@cea.fr.

|August 1, 2014

Summary

This study introduces an automated microfluidic system for single-cell analysis. The electrowetting on dielectric (EWOD) device integrates cell isolation, mRNA purification, and gene expression analysis on one platform.

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Last Updated: Apr 26, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

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Published on: August 3, 2011

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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Area of Science:

Biotechnology
Molecular Biology
Bioengineering

Background:

Single-cell analysis allows for studying cell-to-cell variability, offering new opportunities in life sciences and biomedical research.
Microfluidics and electrowetting on dielectric (EWOD) technology are suitable for small-volume biological studies, including single-cell applications.

Purpose of the Study:

To present the first integrated and automated EWOD-based system for a complete single-cell analysis workflow.
To demonstrate a single-device solution from single-cell isolation to gene expression analysis.

Main Methods:

Development of an integrated microfluidic device utilizing electrowetting on dielectric (EWOD) technology.
Automation of the workflow including single-cell isolation, mRNA purification, and gene expression analysis.

Main Results:

Successful demonstration of a fully automated workflow on a single EWOD device.
Enabling comprehensive analysis from individual cells to gene expression.

Conclusions:

The developed EWOD system provides an efficient and automated platform for single-cell analysis.
This technology advances the potential for detailed investigation of cellular heterogeneity and function.