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Related Experiment Video

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Isolation and Time-Lapse Imaging of Primary Mouse Embryonic Palatal Mesenchyme Cells to Analyze Collective Movement Attributes
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Morphological and molecular changes associated with Pitchfork during mouse palate development.

Chengri Jin1, Jong-Min Lee, Qinghuang Tang

  • 1Department of Orthodontics, Yonsei University College of Dentistry, 50 Yonsei-Ro,, Seodaemun-Gu,, Seoul,, 120-752, Korea.

Cell and Tissue Research
|August 1, 2014
PubMed
Summary

Pitchfork gene over-expression disrupts mammalian palate development by affecting primary cilia disassembly. This impacts Sonic hedgehog signaling and palatine rugae formation, crucial for normal secondary palate morphogenesis.

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Area of Science:

  • Developmental Biology
  • Cell Biology
  • Genetics

Background:

  • Mammalian palate development involves complex cellular and molecular events.
  • Abnormalities in palate development can lead to cleft palate.
  • Ciliary defects are linked to various developmental disorders, including cleft palate.

Purpose of the Study:

  • To investigate the function of the mouse embryonic node gene Pitchfork (Pifo) during palate development.
  • To examine Pifo expression patterns and morphological changes in developing secondary palates upon Pifo over-expression.

Main Methods:

  • Over-expression of Pitchfork in mouse secondary palate cultures.
  • Analysis of Pitchfork expression patterns during embryonic development (E12.5-E13.5).
  • In vitro culture of palates to observe morphological changes.

Main Results:

  • Pitchfork was highly expressed in the developing mouse secondary palate between E12.5 and E13.5.
  • Pitchfork over-expression led to morphological differences in cultured palates compared to controls.
  • Over-expression induced primary cilia disassembly and altered Sonic hedgehog and Patched1 expression levels.

Conclusions:

  • Proper Pitchfork expression levels are critical for normal secondary palate morphogenesis.
  • Pitchfork plays a pivotal role in regulating cellular processes during palate development.
  • The study highlights the link between ciliary function and palate development.