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Protrusion Force Microscopy: A Method to Quantify Forces Developed by Cell Protrusions
Published on: June 16, 2018
Imaging resolution of AFM with probes modified with FIB
J Skibinski1, J Rebis1, T Wejrzanowski1
1Warsaw University of Technology, Faculty of Materials Science and Engineering, ul. Woloska 141, 02-507 Warsaw, Poland.
Abstract:
This study concerns imaging of the structure of materials using AFM tapping (TM) and phase imaging (PI) mode, using probes modified with focused ion beam (FIB). Three kinds of modifications were applied - thinning of the cantilever, sharpening of the tip and combination of these two modifications. Probes shaped in that way were used for AFM investigations with Bruker AFM Nanoscope 8. As a testing material, titanium roughness standard supplied by Bruker was used. The results show that performed modifications influence the oscillation of the probes. In particular thinning of the cantilever enables one to acquire higher self-resonant frequencies, which can be advantageous for improving the quality of imaging in PI mode. It was found that sharpening the tip improves imaging resolution in tapping mode, which is consistent with existing knowledge, but lowered the quality of high frequency topography images. In this paper the Finite Element Method (FEM) was used to explain the results obtained experimentally.
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