Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Functional immune profiling reveals CD4<sup>+</sup> T cell dysregulation in coeliac disease.

Immunology and cell biology·2026
Same author

Cell death in cancer.

Cell·2026
Same author

Non-apoptotic caspase-8 is critical for orchestrating exaggerated inflammation during severe SARS-CoV-2 infection.

Nature communications·2025
Same author

Genome-wide in vivo CRISPR screens identify GATOR1 complex as a tumor suppressor in Myc-driven lymphoma.

Nature communications·2025
Same author

Natriuretic peptides as novel regulators of dendritic cells-mediated inflammation.

Cellular and molecular life sciences : CMLS·2025
Same author

Relative importance of the anti-apoptotic versus apoptosis-unrelated functions of MCL-1 in vivo.

Science (New York, N.Y.)·2025

Related Experiment Video

Updated: Apr 26, 2026

Isolation And Dendritic Cell-Uptake of Small Extracellular Vesicles from Echinococcus granulosus
09:04

Isolation And Dendritic Cell-Uptake of Small Extracellular Vesicles from Echinococcus granulosus

Published on: March 28, 2025

852

Maintaining dendritic cell viability in culture.

David Vremec1, Jacinta Hansen1, Andreas Strasser2

  • 1The Walter and Eliza Hall Institute, 1G Royal Parade, Parkville, Vic. 3052, Australia.

Molecular Immunology
|August 2, 2014
PubMed
Summary

Enhancing dendritic cell (DC) survival in culture is crucial for accurate functional assays. Granulocyte-macrophage colony-stimulating factor improved conventional DC viability, while genetic manipulation, particularly Bcl-2 overexpression, benefited plasmacytoid DCs.

Keywords:
Cytokines and DC survivalDC deathDC death pathwayspDC death

More Related Videos

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
09:09

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

Published on: April 18, 2016

14.9K
Culture of myeloid dendritic cells from bone marrow precursors
06:39

Culture of myeloid dendritic cells from bone marrow precursors

Published on: July 25, 2008

22.3K

Related Experiment Videos

Last Updated: Apr 26, 2026

Isolation And Dendritic Cell-Uptake of Small Extracellular Vesicles from Echinococcus granulosus
09:04

Isolation And Dendritic Cell-Uptake of Small Extracellular Vesicles from Echinococcus granulosus

Published on: March 28, 2025

852
An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
09:09

An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets

Published on: April 18, 2016

14.9K
Culture of myeloid dendritic cells from bone marrow precursors
06:39

Culture of myeloid dendritic cells from bone marrow precursors

Published on: July 25, 2008

22.3K

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Dendritic cells (DCs) exhibit rapid death in culture, compromising functional studies.
  • In vitro DC survival is essential for reliable immunological research and assays.

Purpose of the Study:

  • To identify methods for prolonging the survival of mouse dendritic cells (DCs) in culture.
  • To compare the efficacy of cytokines and genetic manipulation in maintaining DC viability.

Main Methods:

  • Tested various cytokines, including granulocyte-macrophage colony-stimulating factor (GM-CSF), Fms-like tyrosine kinase 3 ligand (Flt3-L), and interleukin-3 (IL-3).
  • Investigated genetic manipulation by culturing DCs from mice overexpressing Bcl-2 or lacking Bim and Noxa.
  • Assessed the viability of conventional DCs (cDCs) and plasmacytoid DCs (pDCs) under different culture conditions.

Main Results:

  • GM-CSF enhanced cDC survival but not pDC survival.
  • Flt3-L and IL-3 provided only marginal improvements in DC survival.
  • Overexpression of Bcl-2 significantly improved pDC viability, with a lesser effect on cDCs.
  • DCs from Bim(-/-)Noxa(-/-) mice showed improved survival, with pDCs benefiting the least.

Conclusions:

  • Cytokine-based strategies show differential effects on DC subsets.
  • Genetic manipulation, particularly Bcl-2 overexpression, offers a promising approach for enhancing pDC survival in culture.
  • Optimizing DC culture conditions is vital for accurate assessment of DC function.