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Nanoparticle/Polymer assembled microcapsules with pH sensing property.

Pan Zhang1, Xiaoxue Song, Weijun Tong

  • 1MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, 310027, China.

Macromolecular Bioscience
|August 2, 2014
PubMed
Summary
This summary is machine-generated.

Researchers developed dual-labeled microcapsules for ratiometric intracellular pH sensing. These nanoparticle/polymer capsules are stable, cell-internalized, and can load drugs, enabling real-time tracking of cellular processes.

Keywords:
cellular uptakeloadingmicrocapsulesnanoparticle/polymer assemblypH sensing

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Cell Biology

Background:

  • Accurate intracellular pH sensing is crucial for understanding cellular functions.
  • Developing stable and cell-compatible sensing platforms remains a challenge.

Purpose of the Study:

  • To fabricate dual-labeled microcapsules for ratiometric intracellular pH sensing.
  • To investigate the stability, cell internalization, and drug-loading capabilities of these microcapsules.

Main Methods:

  • Fabrication of microcapsules using nanoparticle/polymer assembly on polyamine-salt aggregates.
  • Deposition of SiO2 nanoparticles and silicic acid treatment for stability.
  • Simultaneous labeling with pH-sensitive and pH-insensitive dyes for ratiometric sensing.
  • Encapsulation of DNA and dyes via charge interaction.

Main Results:

  • Microcapsules exhibited stability across a wide pH range (3.0–8.0).
  • Positively charged, rough-surfaced microcapsules were readily internalized by cells.
  • In real-time measurements, capsules localized in acidic organelles.
  • Demonstrated ability to encapsulate negatively charged molecules like DNA and dyes.

Conclusions:

  • The developed microcapsules offer a robust platform for ratiometric intracellular pH sensing.
  • Their cell-internalization and drug-loading capacity enable tracking of carrier transport in cells.
  • This technology holds promise for advanced cellular analysis and drug delivery applications.