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Genome analysis of NDM-1 producing Morganella morganii clinical isolate
Abiola Olumuyiwa Olaitan1, Seydina M Diene, Sushim Kumar Gupta
1Unité de recherche sur les maladies infectieuses et tropicales émergentes (URMITE) CNRS-IRD UMR 6236, Méditerranée Infection, Faculté de Médecine et de Pharmacie, Aix-Marseille-Université, Marseille, France.
Objective:
To analyze the resistome and virulence genes of Morganella morganii F675, a multidrug-resistant clinical isolate using whole genome sequencing (WGS).
Methods:
M. morganii F675 was isolated from a patient from Jerusalem, Israel. WGS was performed using both 454 and SOLiD sequencing technologies. Analyses of the bacterial resistome and other virulence genes were performed in addition to comparison with other available M. morganii genomes.
Results:
The assembled sequence had a genome size of 4,127,528 bp with G+C content of 51%. The resistome consisted of 13 antibiotic resistance genes including blaNDM-1 located in a plasmid likely acquired from Acinetobacter spp. Moreover, we characterized for the first time the whole lipid A biosynthesis pathway in this species along with the O-antigen gene cluster, the urease gene cluster and several other virulence genes.
Conclusion:
The WGS analysis of this pathogen further provides insight into its pathogenicity and resistance to antibiotics.
Insights
Whole genome sequencing of Morganella morganii F675 revealed 13 antibiotic resistance genes, including blaNDM-1, and key virulence factors. This analysis deepens our understanding of this multidrug-resistant pathogen and its antibiotic resistance mechanisms.
Area of Science:
- Microbiology
- Genomics
- Infectious Diseases
Background:
- Morganella morganii is an opportunistic pathogen.
- Multidrug resistance in bacteria poses a significant public health threat.
- Understanding the genetic basis of resistance and virulence is crucial for effective treatment.
Purpose of the Study:
- To perform whole genome sequencing (WGS) on a multidrug-resistant clinical isolate of Morganella morganii F675.
- To identify and characterize the resistome and virulence genes of this isolate.
- To compare the genome with other available Morganella morganii genomes.
Main Methods:
- Isolation of Morganella morganii F675 from a clinical sample in Jerusalem, Israel.
- Whole genome sequencing using 454 and SOLiD technologies.
- Bioinformatic analysis of the genome to identify antibiotic resistance genes and virulence factors.
Main Results:
- The assembled genome of M. morganii F675 is 4,127,528 bp with a G+C content of 51%.
- The resistome includes 13 antibiotic resistance genes, notably blaNDM-1 on a plasmid potentially acquired from Acinetobacter spp.
- Characterization of the complete lipid A biosynthesis pathway, O-antigen gene cluster, urease gene cluster, and other virulence genes for the first time in this species.
Conclusions:
- Whole genome sequencing provides valuable insights into the pathogenicity of Morganella morganii.
- The identified genes contribute to the multidrug-resistant phenotype of this clinical isolate.
- Further research into these genetic elements can inform strategies against Morganella morganii infections.
