Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Subcutaneously implanted tissue chambers: a pathophysiological study.

C R Clarke1, C R Short, E A Usenik

  • 1Department of Veterinary Physiology, Pharmacology and Toxicology, School of Veterinary Medicine, Louisiana State University, Baton Rouge 70803.

Research in Veterinary Science
|September 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vulnerability of marine megafauna to global at-sea anthropogenic threats.

Conservation biology : the journal of the Society for Conservation Biology·2025
Same author

To Madagascar and back: long-distance, return migration across open ocean by a pregnant female bull shark Carcharhinus leucas.

Journal of fish biology·2015
Same author

Anatomical and physiological basis for the allometric scaling of cisplatin clearance in dogs.

Journal of veterinary pharmacology and therapeutics·2015
Same author

Penetration of ceftiofur into sterile vs. Mannheimia haemolytica-infected tissue chambers in beef calves after subcutaneous administration of ceftiofur crystalline free acid sterile suspension in the ear pinna.

Journal of veterinary pharmacology and therapeutics·2005
Same author

Effect of dietary fat on oral bioavailability of tepoxalin in dogs.

Journal of veterinary pharmacology and therapeutics·2005
Same author

Comparison of topical lidocaine/prilocaine anesthetic cream and local infiltration of 2% lidocaine for episioplasty in mares.

Journal of veterinary pharmacology and therapeutics·2005
Same journal

Evaluating detection of Histophilus somni immunoglobulin-binding protein A DR2 Fic: A species-specific gene target for recombinase polymerase amplification relative to long-read sequencing of respiratory samples from feedlot calves.

Research in veterinary science·2026
Same journal

Evaluation of bacterial adsorption by ultrapure microporous carbon spheres in dairy cattle: From an in vitro adsorption assay to culture-based assessment of postpartum uterine bacteria.

Research in veterinary science·2026
Same journal

Diagnostic criteria for equine thoracolumbar myofascial pain syndrome: A foundational study.

Research in veterinary science·2026
Same journal

Exploring the prognostic implications of programmed death-ligand 1 expression in canine nodal lymphoma: Insights from surface membrane expression, transcript amount and plasmatic levels.

Research in veterinary science·2026
Same journal

Infrared thermography in cats with squamous cell carcinoma: A pilot study.

Research in veterinary science·2026
Same journal

Reproductive and productive performance in vaccinated and non-vaccinated dairy cows with antibodies against bluetongue virus serotype 3.

Research in veterinary science·2026
See all related articles

Subcutaneous tissue chambers in cattle stabilize by 40 days post-implantation, yielding fluid similar to interstitial fluid. This validated model aids in studying drug distribution in cattle.

Area of Science:

  • Veterinary Science
  • Physiology
  • Pharmacology

Background:

  • Subcutaneous implantation of tissue chambers is a method to study physiological fluid dynamics.
  • Characterizing the evolving composition of fluid within these chambers is crucial for their application in research.

Purpose of the Study:

  • To characterize the temporal changes in tissue and fluid composition within subcutaneous implanted chambers in cattle.
  • To determine the suitability of this model for drug distribution studies.

Main Methods:

  • Subcutaneous implantation of tissue chambers in cattle.
  • Cytological and chemical analysis of chamber fluid over time.
  • Comparison of chamber fluid composition with blood parameters.
  • Histological assessment of chamber tissue vascularity and inflammatory response.

Related Experiment Videos

Main Results:

  • Chamber fluid composition approached true interstitial fluid composition over time.
  • Erythrocyte and leucocyte counts stabilized by 40 days post-implantation.
  • Chamber fluid exhibited lower total protein and albumin, higher K+ and Cl-, and lower pH compared to blood by 40 days.
  • Despite chronic inflammation, chamber tissue vascularity remained stable.

Conclusions:

  • Subcutaneous implanted chambers in cattle provide a stable model for interstitial fluid research by 40 days.
  • The model is suitable for investigating drug distribution due to stabilized cellular and chemical constituents.
  • The chronic inflammatory response does not compromise tissue vascularity essential for drug studies.