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A procedure to obtain long-lasting fluorescence in formaldehyde fixed tissues
M A Moreira1, D Barbieri, L C de Castro
1Faculty of Medicine, Federal University of Goiás, Goiânia, Brasil.
Virchows Archiv. A, Pathological Anatomy and Histopathology
|January 1, 1989
Abstract:
The authors made a study of the small intestine by immunofluorescence in 5% phosphate-buffered formaldehyde fixed tissues, using the Nairn (1976) technique associated with Evans blue (Fry and Wilkinson 1963). The ability of the sections to fluoresce has been maintained after a storage time of more than 4 years. Similar very good results were obtained when skin biopsies from pemphigus foliaceus patients were fixed in formaldehyde for two h and afterwards washed in 30% sucrose solution for 12 to 18 h.