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Solid state fermentation of lipopeptide antibiotic iturin A by using a novel solid state fermentation reactor system
Takashi Ano1, Guang Yuan Jin, Shinji Mizumoto
1Chemical Resources Laboratory, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yoko-hama 226-8503, Japan. tano@res.titech.ac.jp
Journal of Environmental Sciences (China)
|August 2, 2014
Summary
A novel solid-state fermentation reactor (SSFR) optimized iturin A production using Bacillus subtilis. Controlling temperature within 25-30°C significantly improved antibiotic yield, demonstrating SSFR
Area of Science:
- Biotechnology
- Microbial Fermentation
- Bioprocess Engineering
Background:
- Solid-state fermentation (SSF) offers advantages like simplified downstream processing and low cost.
- A new solid-state fermentation reactor (SSFR) was developed for enhanced microbial cultivation.
- Iturin A is a lipopeptide antibiotic with potential applications.
Purpose of the Study:
- To evaluate the efficacy of a new SSFR for iturin A production using Bacillus subtilis.
- To investigate the impact of agitation and temperature control on iturin A yield.
- To assess the feasibility of using okara, a food industry waste, as a substrate.
Main Methods:
- Cultivation of Bacillus subtilis RB14-CS in the SSFR using okara as substrate.
- Comparison of fermentation with and without agitation.
- Monitoring and control of temperature within the reactor.
- Quantification of iturin A production.
Main Results:
- No iturin A production was observed when okara was used with agitation in the SSFR.
- Low iturin A production occurred without agitation due to heat generation and temperature rise.
- Optimal iturin A production was achieved when temperature was maintained between 25-30°C.
- Production levels were comparable to laboratory-scale fermentation.
Conclusions:
- The SSFR shows potential for industrial-scale iturin A production.
- Temperature control is critical for optimizing iturin A yield in SSF.
- Okara can be a suitable substrate for SSF if process parameters are optimized.
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