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The phosphatidylinositol response of chondrocytes in culture
V M Baragi1, D T Dudley, D J Schrier
1Parke-Davis Pharm. Res. Div., Warner-Lambert Co., Ann Arbor, MI 48105.
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|June 1, 1989
Abstract:
This study examined the phosphatidylinositol (PI) response in chondrocytes. The levels of 3H-inositol phosphates (IPs) were measured in guinea pig chondrocyte cultures labelled with 3H-myo-inositol and exposed to various inflammatory mediators and known agonists of the PI response, including bradykinin, carbachol, histamine, A23187, or recombinant human interleukin-1 beta. Of the mediators used to stimulate the PI response in chondrocytes, only bradykinin was effective. The bradykinin treated cells had higher levels of IPs (37-fold) than the controls. The PI response to bradykinin was dose and time dependent.