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GMO testing laboratories can now optimize screening strategies with GMOseek software. This tool enhances cost-efficiency and accuracy in detecting genetically modified organisms (GMOs) in food, feed, and seed samples.

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Area of Science:

  • Agricultural biotechnology
  • Food safety analysis
  • Bioinformatics and computational biology

Background:

  • Increasing commercialization of Genetically Modified Organisms (GMOs) presents challenges for regulatory compliance testing in food, feed, and seed.
  • Laboratories often use a 'matrix approach' to manage resources efficiently but face limitations with existing tools and data utilization.
  • Current methods for GMO testing can be costly and complex, necessitating improved decision-support systems.

Purpose of the Study:

  • To develop and present GMOseek, a freely available software tool designed to optimize GMO screening assay selection for routine laboratory testing.
  • To provide laboratories with a user-friendly interface for cost-efficient GMO testing strategies and decision-making.
  • To support the evaluation of new GMO screening targets and the assessment of new method development profitability.

Main Methods:

  • GMOseek utilizes a matrix of GM events and genetic elements, combined with laboratory analysis history and sample-specific information.
  • An optimization approach suggests the most suitable screening assays for a given sample, allowing user customization for cost-efficiency and multiplexing preferences.
  • The software guides users in selecting analyses for individual GM event detection and aids in final GMO composition determination.

Main Results:

  • GMOseek supports decision-making throughout the GMO testing workflow, from assay selection to result interpretation.
  • The software enables customized, cost-efficient screening assay combinations based on user-defined preferences.
  • Evaluation on real-world food, feed, and seed samples demonstrated GMOseek's superiority over existing tools in GMO coverage and cost-efficiency.

Conclusions:

  • GMOseek offers GMO testing laboratories a powerful, freely accessible tool for selecting optimal assay combinations, including quantitative real-time PCR tests.
  • The software facilitates informed decisions by allowing expert input on multiplexing and cost considerations.
  • GMOseek provides superior GMO coverage and cost-efficiency in screening strategies, simplifying the interpretation of testing results for regulatory compliance.