Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Proteoglycans and vascular cell proliferation.

T N Wight1, S Potter-Perigo, T Aulinskas

  • 1Department of Pathology, School of Medicine, University of Washington, Seattle 98195.

The American Review of Respiratory Disease
|October 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The pharmacokinetics and dosing of oral 4-methylumbelliferone for inhibition of hyaluronan synthesis in mice.

Clinical and experimental immunology·2016
Same author

Differences in the distribution of versican, decorin, and biglycan in atherosclerotic human coronary arteries.

Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology·2015
Same author

Tissue integrity signals communicated by high-molecular weight hyaluronan and the resolution of inflammation.

Immunologic research·2014
Same author

Differential effects of exogenous and endogenous hyaluronan on contraction and strength of collagen gels.

Acta biomaterialia·2008
Same author

Fenofibrate modifies human vascular smooth muscle proteoglycans and reduces lipoprotein binding.

Diabetologia·2004
Same author

Thiazolidinediones reduce the LDL binding affinity of non-human primate vascular cell proteoglycans.

Diabetologia·2004

Proteoglycan (PG) synthesis increases with cell division in monkey aortic smooth muscle cells. Perturbing PG metabolism inhibits proliferation, suggesting PGs may facilitate cell growth.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Vascular Biology

Background:

  • Proteoglycans (PGs) are crucial extracellular matrix components.
  • Their role in regulating cell proliferation, particularly in vascular smooth muscle cells, remains unclear.

Purpose of the Study:

  • To investigate the relationship between proliferative states and proteoglycan synthesis in monkey aortic smooth muscle cells.
  • To determine if proteoglycans play a role in facilitating cell division.

Main Methods:

  • Monkey aortic smooth muscle cells were cultured and made quiescent.
  • Cell proliferation was stimulated using platelet-derived growth factor (PDGF) or 5% serum.
  • [35S]sulfate incorporation was used to measure proteoglycan synthesis.

Related Experiment Videos

Main Results:

  • [35S]sulfate incorporation into proteoglycans increased within 24 hours of growth stimulation, primarily in chondroitin sulfate proteoglycans (CSPGs).
  • Inhibition of proteoglycan metabolism using p-nitrophenyl beta-D-xyloside, heparin, or TGF-beta affected cell proliferation.
  • Elevated proteoglycan synthesis was observed when cell proliferation was inhibited.

Conclusions:

  • Proteoglycan metabolism is modulated by the growth state of arterial smooth muscle cells.
  • While PGs appear involved in cell division, their precise role (direct or indirect) in growth control requires further investigation.