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Preparation of rough microsomes from rat liver

David D Sabatini¹

¹Department of Cell Biology, NYU Medical School, New York, New York 10016.

Cold Spring Harbor Protocols

|August 3, 2014

Abstract:

This protocol describes how to prepare rat liver rough microsomes that contain undegraded membrane-bound polysomes and can function very well in an in vitro translation system. It uses endogenous ribonuclease inhibitor in all steps, avoiding pelleting rough microsomes in all steps and sacrificing good recovery.

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