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Isotype analysis of the anti-CENP-B anticentromere autoantibody: evidence for restricted clonality
R A Eisenberg1, W C Earnshaw, B J Bordwell
1Department of Medicine, University of North Carolina, Chapel Hill.
Arthritis and Rheumatism
|October 1, 1989
Abstract:
Utilizing the centromere B fusion protein (CENP-B) and specific, matched monoclonal antiisotype reagents in an enzyme-linked immunosorbent assay, we found that anti-CENP-B autoantibodies were skewed to the IgG1 isotype. The overall kappa:lambda light chain ratio was 2:1, although several individual sera showed a strong predominance of one of the light chains. Isoelectric focusing of light chain-skewed sera showed polyclonal patterns. Our findings are consistent with the anti-CENP-B autoantibody response being a chronic, antigen-driven response.