Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

4.7K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
4.7K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

4.6K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
4.6K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

7.1K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.1K
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

7.3K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
7.3K
Catenins01:23

Catenins

2.2K
Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
2.2K
cAMP-dependent Protein Kinase Pathways01:25

cAMP-dependent Protein Kinase Pathways

7.3K
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
7.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Break-induced replication is enhanced by a phospho-activated RPA-binding module in Pol32.

Nature communications·2026
Same author

The CIP2A-TOPBP1 axis facilitates mitotic DNA repair via MiDAS and MMEJ.

Nature communications·2025
Same author

DNA2 enables growth by restricting recombination-restarted replication.

Nature·2025
Same author

The balance between B55α and Greatwall expression levels predicts sensitivity to Greatwall inhibition in cancer cells.

Nature communications·2025
Same author

Centromere protection requires strict mitotic inactivation of the Bloom syndrome helicase complex.

Nature communications·2025
Same author

TopBP1 coordinates DNA repair synthesis in mitosis via recruitment of the nuclease scaffold SLX4.

Communications biology·2025

Related Experiment Video

Updated: Apr 26, 2026

Author Spotlight: Integrating BRET-Based Assays and Rare Mutation Analysis to Decipher RAF Kinase Regulation in Live Cells
06:44

Author Spotlight: Integrating BRET-Based Assays and Rare Mutation Analysis to Decipher RAF Kinase Regulation in Live Cells

Published on: March 1, 2024

1.9K

TopBP1: A BRCT-scaffold protein functioning in multiple cellular pathways.

Christopher P Wardlaw1, Antony M Carr1, Antony W Oliver2

  • 1Genome Damage and Stability Centre, School of Life Sciences, University of Sussex, Falmer BN1 9RQ, UK.

DNA Repair
|August 4, 2014
PubMed
Summary
This summary is machine-generated.

TopBP1 is a crucial scaffold protein involved in DNA replication, repair, and checkpoint signaling. Recent studies reveal structural insights into its function and binding partners, with implications for human diseases.

Keywords:
Scaffold proteinsTopBP1 functions in checkpoint signallingTopBP1 functions in replicationTopBP1 review

More Related Videos

Assessing Cellular Target Engagement by SHP2 PTPN11 Phosphatase Inhibitors
08:45

Assessing Cellular Target Engagement by SHP2 PTPN11 Phosphatase Inhibitors

Published on: July 17, 2020

5.7K
Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
10:26

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

Published on: December 20, 2017

10.6K

Related Experiment Videos

Last Updated: Apr 26, 2026

Author Spotlight: Integrating BRET-Based Assays and Rare Mutation Analysis to Decipher RAF Kinase Regulation in Live Cells
06:44

Author Spotlight: Integrating BRET-Based Assays and Rare Mutation Analysis to Decipher RAF Kinase Regulation in Live Cells

Published on: March 1, 2024

1.9K
Assessing Cellular Target Engagement by SHP2 PTPN11 Phosphatase Inhibitors
08:45

Assessing Cellular Target Engagement by SHP2 PTPN11 Phosphatase Inhibitors

Published on: July 17, 2020

5.7K
Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions
10:26

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

Published on: December 20, 2017

10.6K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • TopBP1 is a scaffold protein with nine BRCT domains.
  • It plays vital roles in DNA replication, checkpoint signaling, DNA repair, and transcriptional control.
  • Numerous publications have highlighted TopBP1's importance since 2005.

Purpose of the Study:

  • To review recent advancements in understanding TopBP1 function.
  • To emphasize structural insights into TopBP1's mechanisms.
  • To summarize TopBP1's binding partners and its links to human disease.

Main Methods:

  • Literature review of recent scientific publications.
  • Analysis of structural data related to TopBP1.
  • Identification and summary of TopBP1's binding partners.

Main Results:

  • TopBP1 acts as a key scaffold, linking components in DNA replication, checkpoint signaling, DNA repair, and transcription.
  • Recent structural studies provide new insights into TopBP1's functional mechanisms.
  • Several binding partners involved in these processes have been identified.

Conclusions:

  • TopBP1 is a central regulator of critical cellular processes.
  • Structural understanding is advancing our knowledge of TopBP1's multifaceted roles.
  • TopBP1's dysfunction is linked to various human diseases.