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Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Potential of multi-photon upconversion emissions for fluorescence diffuse optical imaging.

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    Rare-earth-doped upconverting nanoparticles enhance spatial resolution in diffuse optical imaging. Utilizing multi-photon upconversion emissions significantly improves imaging clarity for biomedical applications.

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    Area of Science:

    • Biomedical Optics
    • Nanotechnology
    • Materials Science

    Background:

    • Spatial resolution is crucial for fluorescence molecular imaging.
    • Multi-photon emissions offer a pathway to enhance imaging resolution.
    • Rare-earth-doped upconverting nanoparticles (UCNPs) are promising for optical imaging.

    Purpose of the Study:

    • To investigate the potential of multi-photon upconversion emissions from UCNPs for improving spatial resolution in diffuse optical imaging.
    • To quantify the resolution enhancement achieved by two-photon and three-photon upconversion emissions.
    • To explore pulsed excitation for overcoming low quantum yield in high-order upconversion.

    Main Methods:

    • Utilizing rare-earth-doped upconverting nanoparticles.
    • Employing multi-photon upconversion emissions (two-photon and three-photon).
    • Comparing resolution with linear emission under pulsed excitation.

    Main Results:

    • Imaging resolution improved by a factor of 1.45 using two-photon upconversion compared to linear emission.
    • Further resolution enhancement by a factor of 1.23 achieved with three-photon upconversion.
    • Pulsed excitation demonstrated to mitigate low quantum yield issues in high-order upconversion.

    Conclusions:

    • Multi-photon upconversion emissions from UCNPs significantly enhance spatial resolution in diffuse optical imaging.
    • Higher-order photon emissions offer greater resolution improvements.
    • Pulsed excitation is a viable strategy for practical application of high-order upconversion in imaging.