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Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules

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Ultrafast 2D IR microscopy.

Carlos R Baiz, Denise Schach, Andrei Tokmakoff

|August 5, 2014

Summary

We developed a novel microscope for detailed analysis of molecular vibrations in heterogeneous samples. This tool provides high-resolution imaging of molecular dynamics using two-dimensional infrared spectroscopy.

Area of Science:

Chemical Physics
Spectroscopy
Materials Science

Background:

Heterogeneous samples present challenges for molecular analysis.
Understanding vibrational dynamics is crucial for materials characterization.
Existing methods lack sufficient spatial and temporal resolution.

Purpose of the Study:

To introduce a new microscope for measuring two-dimensional infrared (2D IR) spectra.
To achieve μm-scale spatial and sub-picosecond time resolution for vibrational dynamics.
To enable imaging of microscopic variations in molecular environments.

Main Methods:

Utilizing a fully collinear "one beam" optical geometry.
Employing polarization, chopping, and phase cycling for signal isolation.

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Leveraging two-dimensional infrared (2D IR) spectroscopy for molecular structure information.

Main Results:

Demonstrated μm-scale spatial and sub-picosecond time resolution.
Successfully measured vibrational dynamics through frequency, time, and space correlations.
Showcased vibrational lifetime as a contrast agent for microscopic imaging.

Conclusions:

The developed microscope offers unprecedented capabilities for analyzing heterogeneous samples.
This technique provides insights into molecular environments and dynamics.
Vibrational lifetime imaging opens new avenues for materials science research.