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Long noncoding RNA expression profiles of lung adenocarcinoma ascertained by microarray analysis
Gang Xu1, Jie Chen2, Qinshi Pan1
1Department of Laboratory Medicine, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
Plos One
|August 5, 2014
Summary
This study identified numerous differentially expressed long noncoding RNAs (lncRNAs) in lung adenocarcinoma tissues. These findings suggest lncRNAs play a significant role in lung cancer development.
Area of Science:
- Molecular Biology
- Genomics
- Oncology
Background:
- Long noncoding RNAs (lncRNAs) are implicated in lung cancer development and progression.
- The specific roles of lncRNAs in lung cancer remain largely uncharacterized.
Purpose of the Study:
- To investigate the expression patterns of lncRNAs in lung adenocarcinoma.
- To identify lncRNAs associated with lung adenocarcinoma development.
Main Methods:
- High-throughput microarray analysis of lncRNA and mRNA expression profiles in lung adenocarcinoma and normal tissues.
- Verification of candidate lncRNAs using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR).
Main Results:
- Microarray analysis revealed differential expression of 2,420 lncRNAs and 1,109 mRNAs (≥2-fold change) in lung adenocarcinoma compared to normal tissue.
- qRT-PCR confirmed aberrant expression of 19 lncRNAs in lung adenocarcinoma tissues.
- LOC100132354 and RPLP0P2 were identified as the most aberrantly expressed lncRNAs in 100 patient samples.
Conclusions:
- The study successfully profiled lncRNA expression in lung adenocarcinoma.
- The differential expression of numerous lncRNAs suggests their critical involvement in lung cancer pathogenesis.
