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[Optimization of enzymatic extraction of polysaccharide from Dendrobium officinale by box-Behnken design and response
Objective:
To optimize the processing of enzymatic extraction of polysaccharide from Dendrobium officinale.
Methods:
With phenol-sulfuric acid method and the DNS determination polysaccharide, Box-Behnken response surface methodology was used to optimize different enzyme dosage, reaction temperature and reaction time by using Design-Expert 8.05 software for data analysis and processing.
Results:
According to Box-Behnken response, the best extraction conditions for the polysaccharide from Dendrobium officinale were as follows: the amount of enzyme complex was 3.5 mg/mL, hydrolysis temperature was 53 degrees C, and reaction time was 70 min. In accordance with the above process, the polysaccharide yield was 16.11%.
Conclusion:
Box-Behnken response surface methodology is used to optimize the enzymatic extraction process for the polysaccharide in this study, which is effective, stable and feasible.
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