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Updated: Apr 26, 2026

A Rapid In Vivo Bioassay for Developmentally Active Enhancers
28-Homobrassinolide: a novel oxysterol transactivating LXR gene expression
R Premalatha1, K Srikumar, D Vijayalaksmi
1Department of Biochemistry & Molecular Biology, Pondicherry University, Pondicherry, 605014, India, prema.srikumar@gmail.com.
Abstract:
Cholesterol is the template for steroid hormone biosynthesis. Cholesterol homeostasis is regulated by Cyt-P450 oxygenated cholesterols acting as ligands on LXR-α and LXR-β transcription factors that are now emerging as drug targets. Heterodimerization of LXRs with retinoic acid receptor is considered a prerequisite for target gene activation. Dietary plant oxysterol 28-homobrassinolide (28-HB) is a proven antihyperglycemic and a pro-steroidogenic agent in the rat. Whether 28-HB has a role in LXR gene expression was therefore investigated using oral gavage (15 days) of 28-HB (333 µg/kg b w) to normal and diabetic rat. PCR amplified LXR-α and β mRNA transcripts from treated rat liver and testis exhibited quantitative differences in their expression. Conformational differences in 28-HB docking to LXR-α and β binding domains were also noted through in silico studies, LXR-β adopting lesser specificity. We report that 28-HB transactivates LXR genes in the rat tissues.
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