Effect of various doses of FSH upon luteal cell function in tissue culture

Luteal cells were collected from porcine ovaries in early or midluteal phase of the cycle. The cells were cultured as monolayers for 2 days. The culture medium except that of a control one was supplemented with either 1, 10, 100 or 500 ng of FSH (NIH-FSH-S-8 OVINE) or 1, 10, 100 or 500 ng of FSH pretreated with antiserum anti-LH. Progesterone secretion by the cultures of luteal cells collected from early corpora lutea. However was stimulated by FSH in a dose-response manner when FSH was pretreated with anti-LH serum such progesterone secretion was stimulated to a lesser, although significant (P less than 0.05) extent. In contrast, FSH suppressed the secretion of estradiol by the cultures of the cells of early luteal phase, while the cells collected from the corpora lutea of midluteal phase did not respond to FSH stimulation.