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Related Experiment Videos

Automated amidolytic method for evaluating the activated partial thromboplastin time compared with a conventional

A Tripodi1, M Poggio, L Scapellato

  • 1A. Bianchi Bonomi Hemophilia and Thrombosis Center, University of Milan, Italy.

Haemostasis
|January 1, 1989
PubMed
Summary

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A new amidolytic method for activated partial thromboplastin time (APTT) testing shows good correlation with the clotting method for most coagulation disorders. However, it is less sensitive to heparin and lupus anticoagulants.

Area of Science:

  • Hematology
  • Clinical Chemistry
  • Coagulation Diagnostics

Background:

  • The activated partial thromboplastin time (APTT) is a crucial test for evaluating the intrinsic coagulation pathway.
  • Conventional clotting methods for APTT assessment can be influenced by various factors, necessitating exploration of alternative techniques.

Purpose of the Study:

  • To investigate the clinical validity and application of a novel amidolytic method for APTT measurement.
  • To compare the performance of the amidolytic APTT method against a standard clotting method across diverse patient plasma samples.

Main Methods:

  • Comparative analysis of an amidolytic APTT assay versus a conventional clotting APTT assay.
  • Testing involved normal plasma, plasma from hemophilia patients, and plasma from individuals undergoing heparin therapy.

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  • Evaluation included assessment of congenital deficiencies and detection of lupus anticoagulants.

    • Main Results:

    • Strong correlation between amidolytic and clotting APTT methods for normal plasma and hemophilia patient plasma.
    • Congenital intrinsic pathway deficiencies (excluding fibrinogen issues) were consistently detected by both methods.
    • The amidolytic method exhibited reduced sensitivity to heparinization compared to the clotting method.
    • The amidolytic method could quantify Factor VIII and IX levels when used with deficient plasmas.
    • Detection of lupus anticoagulants was less sensitive with the amidolytic method than with the clotting method.

    Conclusions:

    • The amidolytic APTT method offers a valid alternative to clotting assays for routine coagulation screening and specific factor assays.
    • Clinical utility is demonstrated for diagnosing hemophilia and certain intrinsic pathway defects.
    • Limitations include decreased sensitivity to heparin and lupus anticoagulants, requiring careful interpretation in specific clinical contexts.