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Updated: Aug 11, 2026

Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons
Published on: February 12, 2014
Intracellular and surface distribution of a membrane protein (CD8) derived from a single nucleus in multinucleated
1Department of Physiology, School of Medicine, University of California, San Francisco 94143-0444.
Abstract:
We have investigated the contribution of an individual nucleus to intracellular and surface membranes in multinucleated muscle fibers. Using a retroviral vector, we introduced the gene encoding the human T-lymphocyte antigen CD8 into C2 mouse muscle cells to form a stable line expressing the human protein on its surface. The intracellular and surface distributions of the protein were then investigated by immunocytochemistry in hybrid myotubes containing a single nucleus expressing CD8. We show that the intracellular distribution of CD8 is limited to a local area surrounding the nucleus encoding it and several neighboring nuclei. On the cell surface, however, the protein is distributed over the entire myotube. Widespread distribution of a surface membrane protein in multinucleated myotubes can thus result from localized synthesis and processing.
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