Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic modification of the marine-derived yeast Yarrowia lipolytica with high-protein content using a GPI-anchor-fusion expression system.

Biotechnology progress·2009
Same author

Regulation of the Edwardsiella tarda hemolysin gene and luxS by EthR.

Journal of microbiology and biotechnology·2009
Same author

EBV LMP2A-specific T cell immune responses elicited by dendritic cells loaded with LMP2A protein.

Cellular & molecular immunology·2009
Same author

[Combination of volar buttress plate with external fixator for the distal radial fractures of type C3 caused by high-energy injuries].

Zhongguo gu shang = China journal of orthopaedics and traumatology·2009
Same author

Environmental regulation of floral anthocyanin synthesis in Ipomoea purpurea.

Molecular ecology·2009
Same author

PI3K integrates the effects of insulin and leptin on large-conductance Ca2+-activated K+ channels in neuropeptide Y neurons of the hypothalamic arcuate nucleus.

American journal of physiology. Endocrinology and metabolism·2009

Related Experiment Video

Updated: Apr 26, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

2.2K

[Performance of wavelength modulation surface plasmon resonance biosensor].

Yun-Han Luo, Meng-Yun Xu, Xiao-Long Chen

    Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
    |August 7, 2014
    PubMed
    Summary

    This study developed a Kretschmann-structure surface plasmon resonance (SPR) biosensor. The SPR biosensor demonstrates high sensitivity and a wide detection range for refractive index changes, making it suitable for chemical and biological sensing applications.

    More Related Videos

    Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons
    07:39

    Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

    Published on: July 21, 2018

    6.5K
    Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum
    09:23

    Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum

    Published on: December 13, 2017

    5.9K

    Related Experiment Videos

    Last Updated: Apr 26, 2026

    Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
    06:12

    Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

    Published on: March 17, 2023

    2.2K
    Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons
    07:39

    Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons

    Published on: July 21, 2018

    6.5K
    Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum
    09:23

    Using Extraordinary Optical Transmission to Quantify Cardiac Biomarkers in Human Serum

    Published on: December 13, 2017

    5.9K

    Area of Science:

    • Optoelectronics
    • Biosensing Technology
    • Analytical Chemistry

    Background:

    • Surface plasmon resonance (SPR) is a key technique for label-free, high-precision biological analysis.
    • Understanding SPR characteristics is crucial for advancing its applications in sensing.
    • Kretschmann-structure SPR biosensors offer a platform for sensitive detection.

    Purpose of the Study:

    • To develop and investigate the sensing performance of a Kretschmann-structure SPR biosensor.
    • To analyze the sensor's response to varying refractive indices in bulk solutions.
    • To establish the theoretical basis and practical guidelines for SPR biosensor applications.

    Main Methods:

    • Development of a Kretschmann-structure SPR biosensor utilizing wavelength modulation.
    • Experimental investigation of the biosensor's performance using ethanol and ethylene glycol solutions of varying concentrations.
    • Characterization of resonant wavelength shifts in response to changes in refractive index (RI).

    Main Results:

    • The SPR biosensor exhibited a low sensitivity at lower RI values but a higher linear response.
    • Sensitivity increased with rising refractive index, reaching 11,487 nm/RIU in the 1.4070-1.430 RIU range.
    • The sensor demonstrated excellent stability (0.2138 nm) and a minimum RI resolution of 10^-6 RIU.

    Conclusions:

    • The developed SPR biosensor offers a simple operation, high sensitivity, wide detection range, and low resolution.
    • Its performance characteristics make it a strong candidate for advanced chemical and biological sensing.
    • This research provides valuable insights for the practical application and further development of SPR-based sensing technologies.