Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Translesion DNA Polymerases02:10

Translesion DNA Polymerases

9.3K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
9.3K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

8.5K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
8.5K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

2.3K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

177Lu-based radioligand therapy: A retrospective multicenter analysis to calculate the effective half-life and follow-up dose for the public.

Nuklearmedizin. Nuclear medicine·2026
Same author

Engineering of High-Yield Recombinant Adeno-Associated Virus Producer Plasmids.

Biotechnology journal·2026
Same author

The German Cancer Consortium (DKTK) multi-center prospective phase 1/2 <sup>68</sup>Ga-PSMA-11 PET-imaging trial in newly-diagnosed high-risk prostate cancer: Safety and diagnostic accuracy compared to histopathology and their impact on patient management.

European journal of nuclear medicine and molecular imaging·2025
Same author

Different <sup>212</sup>Pb Generators and Its Radiation Safety Concerning <sup>220</sup>Rn (Thoron) Emanation.

Toxics·2025
Same author

[<sup>203/212</sup>Pb]Pb-VMT-α-NET as a novel theranostic agent for targeted alpha radiotherapy-first clinical experience.

European journal of nuclear medicine and molecular imaging·2025
Same author

Comparison of ZnS(Ag) Scintillator and Proportional Counter Tube for Alpha Detection in Thin-Layer Chromatography.

Pharmaceuticals (Basel, Switzerland)·2025

Related Experiment Video

Updated: Apr 26, 2026

A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells
11:58

A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells

Published on: March 5, 2018

9.9K

99mTc-labeled HYNIC-DAPI causes plasmid DNA damage with high efficiency.

Joerg Kotzerke1, Robert Punzet1, Roswitha Runge1

  • 1University Hospital/Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Department of Nuclear Medicine, Dresden, Germany.

Plos One
|August 8, 2014
PubMed
Summary

Technetium-99m labeled HYNIC-DAPI causes significantly more DNA damage, including double-strand breaks, than pertechnetate. This indicates direct DNA interaction, unlike the radical-induced damage from pertechnetate.

More Related Videos

A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage
07:57

A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage

Published on: May 10, 2022

6.6K
Author Spotlight: Combining Proximity Ligand Assay with Gamma-H2AX Staining to Characterize Protein Interactions in DNA Damage Response
09:39

Author Spotlight: Combining Proximity Ligand Assay with Gamma-H2AX Staining to Characterize Protein Interactions in DNA Damage Response

Published on: August 2, 2024

1.2K

Related Experiment Videos

Last Updated: Apr 26, 2026

A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells
11:58

A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells

Published on: March 5, 2018

9.9K
A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage
07:57

A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage

Published on: May 10, 2022

6.6K
Author Spotlight: Combining Proximity Ligand Assay with Gamma-H2AX Staining to Characterize Protein Interactions in DNA Damage Response
09:39

Author Spotlight: Combining Proximity Ligand Assay with Gamma-H2AX Staining to Characterize Protein Interactions in DNA Damage Response

Published on: August 2, 2024

1.2K

Area of Science:

  • Nuclear medicine
  • Radiochemistry
  • Molecular biology

Background:

  • Technetium-99m ((99m)Tc) is a key radionuclide in nuclear medicine imaging.
  • (99m)Tc emits low-energy electrons, posing a risk for DNA damage.
  • Understanding DNA interaction is crucial for radiopharmaceutical safety and efficacy.

Purpose of the Study:

  • To compare the plasmid DNA damage potential of (99m)Tc-HYNIC-DAPI with (99m)Tc pertechnetate ((99m)TcO4(-)).
  • To investigate the mechanisms of DNA damage induced by these (99m)Tc compounds.

Main Methods:

  • Irradiation of pUC19 plasmid DNA with (99m)Tc-HYNIC-DAPI or (99m)TcO4(-) for 2 or 24 hours.
  • Evaluation of direct and radical-induced DNA damage using gel electrophoresis.
  • Assessment of single-strand breaks (SSBs) and double-strand breaks (DSBs) in the presence or absence of DMSO (radical scavenger).

Main Results:

  • Both compounds increased DNA damage with higher activity, evidenced by increased open circular and linear DNA fractions.
  • (99m)Tc-HYNIC-DAPI induced approximately twice the SSBs and fivefold more DSBs compared to (99m)TcO4(-).
  • DMSO significantly reduced damage from (99m)TcO4(-) but had a minimal effect on (99m)Tc-HYNIC-DAPI, indicating direct DNA interaction.

Conclusions:

  • (99m)Tc-HYNIC-DAPI directly interacts with DNA, causing SSBs and DSBs.
  • (99m)TcO4(-) primarily induces SSBs via radical formation, with DSBs resulting from adjacent SSBs.
  • (99m)Tc-HYNIC-DAPI exhibits significantly higher biological effectiveness in inducing DNA damage compared to (99m)TcO4(-).