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The human lymphocyte micronucleus assay: a comparison between whole-blood and separated-lymphocyte cultures
L Migliore1, M Nieri, S Amodio
1Dipartimento di Scienze dell'Ambiente e del Territorio, Università di Pisa, Italy.
Mutation Research
|November 1, 1989
Abstract:
The induction of micronuclei (MN) by vincristine, mitomycin C and cyclophosphamide was compared in purified lymphocytes and in whole-blood cultures. With both assays, cytokinesis was blocked by cytochalasin B and MN were only scored in binucleate cells. The data suggest that whole-blood cultures may be considered a better experimental condition for the detection of MN induced by chemicals in vitro.